Valeur diagnostique de deux tests rapides utilisés dans le diagnostic du VIH-2 au Mali / Diagnosis value of two HIV-2 rapid diagnostic tests used in Mali

Context and objective. In sub-Saharan Africa, the epicenter of HIV infection, rapid tests are proposed in first line, but diagnosis value of these tests is
rarely performed. The goal of the present study was to evaluate the performance of 2 rapid tests used for the diagnosis of HIV-2 infection
compared to a baseline test in order to propose in algorithm for HIV infection diagnosis in health care system.

Methods. A cross-sectional study
was carried out in three treatment centers in Mali (Bamako, Segou and Sikasso). The tests evaluated were: Genie® II HIV-1/HIV-2 (Sanofi Diagnostic
Pasteur, France) and ImmunoComb® II HIV 1&2 BiSpot (Organics, Strasbourg, France). The study involved 34 sera collected consecutively. The
l’INNO-LIA HIV I/II Score confirmatory test was used as a reference test in Belgium. Performance of tests were assessed using sensibility, specificity,
positive predictive value, negative predictive value and kappa concordance. Results. Patients’ ages ranged from 12 years to 78 years, 19 patients were
women (55.8%) and 28 patients had HIV-2 infection. The sensitivity of the rapid tests was 96.4%. The kappa concordance coefficient was
0.85. We found 28 HIV-2 positive patients out of 34 patients.

Conclusions. The two rapid tests used in Mali yielded satisfactory results, but the quality
of HIV-1 and 2 discrimination serology could be improved.
Key words: HIV-2 diagnosis, ImmunoComb II, Genie II, l’INNO-LIA, Mali
Received: December 1st, 2018
Accepted: May 22th, 2019

Context and objective. In sub-Saharan Africa, the epicenter of HIV infection, rapid tests are proposed in first line, but diagnosis value of these tests is
rarely performed. The goal of the present study was to evaluate the performance of 2 rapid tests used for the diagnosis of HIV-2 infection
compared to a baseline test in order to propose in algorithm for HIV infection diagnosis in health care system.

Methods. A cross-sectional study was carried out in three treatment centers in Mali (Bamako, Segou and Sikasso). The tests evaluated were: Genie® II HIV-1/HIV-2 (Sanofi Diagnostic
Pasteur, France) and ImmunoComb® II HIV 1&2 BiSpot (Organics, Strasbourg, France). The study involved 34 sera collected consecutively. The
l’INNO-LIA HIV I/II Score confirmatory test was used as a reference test in Belgium. Performance of tests were assessed using sensibility, specificity,
positive predictive value, negative predictive value and kappa concordance. Results. Patients’ ages ranged from 12 years to 78 years, 19 patients were
women (55.8%) and 28 patients had HIV-2 infection. The sensitivity of the rapid tests was 96.4%. The kappa concordance coefficient was
0.85. We found 28 HIV-2 positive patients out of 34 patients.

Conclusions. The two rapid tests used in Mali yielded satisfactory results, but the quality
of HIV-1 and 2 discrimination serology could be improved.
Key words: HIV-2 diagnosis, ImmunoComb II,
Genie II, l’INNO-LIA, Mali
Received: December 1st, 2018
Accepted: May 22th, 2019